Bio Effect Analysis
Real-time PCR (Real-time polymerase chain reaction, 실시간 중합효소연쇄반응)중앙공동기기센터
| 장비 한글명 | 실시간 중합효소연쇄반응 | Equipment | RT-PCR (Real-time polymerase chain reaction) |
|---|---|---|---|
| Model | Light Cycle 96 | Maker | Roche |
| Purchase Year | 2017 | Location | Room 722, NIX |
| Researcher | Jeong Wan Park | Tel | e-mail : jwpark0801@suwon.ac.kr Tel.: 031-229-8666 |
| Fees | Inside University Use: 20,000 won per time/ 20,000 plate Outside University Use: 40,000 won per time/ 20,000 won per plate |
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| Application Field | - Quantification of DNA and RNA, Analysis of expression pattern of microRNA | ||
| Principles and Characteristics | It applies a combined probe between a primer and fluorescence dye to the target DNA sequence, amplifies PCR and measures the degree of fluorescence. It then reveals the presence of the target DNA and the amplification of DNA in quantity simultaneously. | ||
| Standard Features |
■ Plate-based real-time PCR system with 96-well block ■ Temperature gradient ■ 4-plex multicolor ■ Runtime of less than 1 hour ■ Applications ■ Absolute Quantification, Relative Quantification, Endpoint Genotyping, ■ Melting Curve Analysis |
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Chemi Doc (젤 이미지시스템)중앙공동기기센터
| 장비 한글명 | 젤이미지시스템 | Equipment | Chemi Doc |
|---|---|---|---|
| Model | ChemiDoc-It2 | Maker | UVP Inc. |
| Purchase Year | 2016 | Location | Room 722, NIX |
| Researcher | Jeong Wan Park | Tel | e-mail : jwpark0801@suwon.ac.kr Tel.: 031-229-8666 |
| Fees | Inside University Use: 5,000 won per figure Outside University Use: 10,000 won per figure |
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| Application Field |
■ Chemiluminescence ■ Western, Nothern, Southern Blots ■ Fluorescent Gels ■ Colorimetric Gels |
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| Principle and Characteristics | It is an equipment to obtain its image using CCD camera, when Chemiluminescence is generated by treating ECL with Gel or Membrane. | ||
| Basic specification: |
■ Up to 7.3 megapixels with a base of 2.1 million pixels ■ Zoom lens: Electric or manual control available ■ 4 types of filter can be installed (EtBr filter is installed as standard) ■ Transmitted light source: UV ■ Reflected light source: white light |
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Absorbance Microplate Readers (마이크로플레이트리더)중앙공동기기센터
| 장비 한글명 | 마이크로플레이트리더 | Equipment name | Absorbance Microplate Readers |
|---|---|---|---|
| Model | Epoch (VT05404-0998) | Producer | BioTek |
| Purchase year | 2016 | Installation location | # 722 NIX(Next Innovation Xroard) |
| Manager | Jungwan Park | Contact | e-mail : jwpark0801@suwon.ac.kr Tel : 031-229-8666 |
| Fees | Inside basic charge: 10,000, 6,000/plate Outside basic charge: 10,000, 10,000/plate |
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| Field of application |
■ ELISA ■ Enzyme Kinetics ■ Nucleic acid quantification ■ Cell proliferation ■ Cytotoxicity |
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| Principle and characteristic | Light from a light source is monochromated by a monochromatic spectral light source and is transmitted through a sample solution to convert the intensity of transmitted light into an electrical signal to measure the absorbance of the sample. | ||
| Basic specification |
■ Wavelength : 200 – 999 nm ■ Detection modes : Absorbance ■ Read methods : Endpoint, Kinetic, Spectral scanning, Well area scanning ■ Microplate types : 6- to 384-well plates ■ Shaking : Not available |
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